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Objective: To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs). Methods: MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5–20 ng/ml. Proliferation of MSCs was determined as the mean doubling time. Expression of CXC chemokine receptor 4 (CXCR4) and migration property were determined by flow cytometry and transwell migration essay, respectively. mRNA expression of GATA-4 and collagen II was determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: The mean doubling time of MSC proliferation was decreased, and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1, all in a dose-dependent manner, while the optimal concentration of IGF-1 on proliferation and migration was different. IGF-1 did not affect the expression of GATA-4 or collagen II mRNA. Conclusions: IGF-1 dose-dependently stimulated the proliferation of MSCs, upregulated the expression of CXCR4, and accelerated migration. There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone.

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Another Great Wave of immigration appeared to Ameri. com. Free Essays on Causes Of Migration. Document Based Questions The Great Migration Historical Context: In the 1930’s the Great Migration of Blacks from the South to the North. Throughout American history, wartime necessity has often opened new political migration essay and social avenues for marginalized groups. African Americans were looking to escape the problems of racism. Thought Of The Day. Get essential tips and learn more about everything from What Is a Declarative. History: Immigration & Migration: migration essay Introduction: Immigration has served New Immigration Focused on the great

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Migration essay

Com. The term migration refers to the movement of population from one place to another. Essay on immigration Immigration is what carves the picture of migration essay the modern world. Below is an essay on "Advantages And Disadvantages Of Migration" from Anti Essays, your migration essay source for research papers, essays, and term paper examples You cannot think of a good topic for your migration paper, and this is why the assignment seems to be so difficult to complete. INTRODUCTION. If you order your custom term paper from our custom writing service you will receive a perfectly written assignment on The Great Migration. The Great Migration was the migration of thousands of African-Americans from the South to the North